Article
Typing of human papillomavirus by pyrosequencing.
Laboratory investigation; a journal of technical methods and pathology - 1 May 2001
Gharizadeh B, Kalantari M, Garcia C A, Johansson B, Nyrén P
Abstract excerpt
The possibility of using a new bioluminometric DNA sequencing technique, called pyrosequencing, for typing of human papillomaviruses (HPV) was investigated. A blinded pyrosequencing test was performed on an HPV test panel of 67 GP5+/GP6+ PCR-derived amplification products. The 67 clinical DNA samples were sequenced up to 25 bases and sequences were searched using BLAST. All of the samples were correctly genotyped...
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