Article
A novel method for multiplex genotyping in a single reactor using GTPlex-PyroSeq: genotyping HPV as a prototype.
The Journal of molecular diagnostics : JMD - 1 Jul 2012
Oh Myungsok, Hoehn Benjamin Douglass, Moon Youngho, Oh Taejeong, Ko Youngbok, An Sungwhan
Abstract excerpt
Herein, we describe a novel multiplex genotyping method, GTPlex-PyroSeq. This method consists of two phases: multiplex PCR followed by a single reaction of pyrosequencing. This study demonstrates how GTPlex-PyroSeq can be adapted for the determination of multiple human papillomavirus (HPV) genotypes. A biotinylated consensus primer, GP6+, and 15 high-risk HPV type-specific primers are used for multiplex PCR. Each...
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