Article
High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli.
Applied microbiology and biotechnology - 1 Aug 2010
Wang Dan, Li Qiang, Mao Yu, Xing Jianmin, Su Zhiguo
Abstract excerpt
Escherichia coli strains with foreign genes under the isopropyl-beta-D: -thiogalactopyranoside-inducible promoters such as lac, tac, and trc were engineered and considered as the promising succinic acid-producing bacteria in many reports. The promoters mentioned above could also be induced by lactose, which had not been attempted for succinic acid production before. Here, the efficient utilization of lactose as...
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