Article
Characterization of sialyltransferase mutants using surface plasmon resonance.
Glycobiology - 1 Mar 2001
Laroy W, Ameloot P, Contreras R
Abstract excerpt
Sialyltransferases are enzymes responsible for the important sialylation of glycoconjugates. Since crystal structures are not available, other tools are needed to study enzymatic mechanisms. As a model, we used human alpha2,6-sialyltransferase. A putative acceptor-binding domain containing the small and the very small sialyl motifs was randomly mutated. This resulted in enzymes with altered enzymatic activity....
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