Automated on-chip analysis of tuberculosis drug-resistance mutation with integrated DNA ligation and amplification.

Abstract excerpt

Detection of a single base mutation in Mycobacterium tuberculosis DNA can provide fast and highly specific diagnosis of antibiotic-resistant tuberculosis. Mutation-specific ligation of padlock probes (PLPs) on the target followed by rolling circle amplification (RCA) is highly specific, but challenging to integrate in a simple microfluidic device due to the low temperature stability of the phi29 polymerase and...

Topics

Join the communities discussing this publication.

• DNA, Bacterial
• Lab-On-A-Chip Devices
• Limit of Detection
• Mutation
• Mycobacterium tuberculosis