Article
Quantification of growth hormone receptor extra- and intracellular domain gene expression in chicken liver by quantitative competitive RT-PCR.
General and comparative endocrinology - 1 May 2001
Van As P, Janssens W, Onagbesan O M, Bruggeman V, Buys N, Sanders J, Van Der Geyten S, Darras V M, Decuypere E
Abstract excerpt
The very sensitive competitive reverse transcription-polymerase chain reaction (RT-PCR) was used to investigate the expression of the extracellular (GHRe) and intracellular (GHRi) parts of the growth hormone receptor (GHR) in the liver tissue of chickens. Two competitors (internal standards), pGHRi MUT and pGHRe MUT, specific to the GHRi and GHRe genes, respectively, were constructed by site-specific mutagenesis....
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