Article
Fish growth hormone receptor: molecular characterization of two membrane-anchored forms.
Endocrinology - 1 Jul 2001
Calduch-Giner J, Duval H, Chesnel F, Boeuf G, Pérez-Sánchez J, Boujard D
Abstract excerpt
A RT-PCR approach was used to clone and sequence the full-length growth hormone receptor (GHR) of a teleost fish, the turbot (Scophthalmus maximus). Total liver RNA was amplified by RT-PCR with degenerate primers designed in extracellular and cytoplasmic regions, and a single DNA fragment of 1100 bp was obtained. The entire coding region was obtained by 5' and 3' RACE assays, and comprises an open-reading frame...
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