Article
Enlarging the amino acid set of Escherichia coli by infiltration of the valine coding pathway.
Science (New York, N.Y.) - 20 Apr 2001
Döring V, Mootz H D, Nangle L A, Hendrickson T L, de Crécy-Lagard V, Schimmel P, Marlière P
Abstract excerpt
Aminoacyl transfer RNA (tRNA) synthetases establish the rules of the genetic code by catalyzing the aminoacylation of tRNAs. For some synthetases, accuracy depends critically on an editing function at a site distinct from the aminoacylation site. Mutants of Escherichia coli that incorrectly charge tRNA(Val) with cysteine were selected after random mutagenesis of the whole chromosome. All mutations obtained were...
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