Article
Identification of inhibitor binding sites of the cAMP-specific phosphodiesterase 4.
Cellular signalling - 1 Apr 2001
Richter W, Unciuleac L, Hermsdorf T, Kronbach T, Dettmer D
Abstract excerpt
Using the technique of site-directed mutagenesis, point mutants of human PDE4A have been developed in order to identify amino acids involved in inhibitor binding. Relevant amino acids were selected according to a peptidic binding site model for PDE4 inhibitors, which suggests interaction with two tryptophan residues, one histidine and one tyrosine residue, as well as one Zn(2+) ion. Mutations were directed at...
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