Article
cAMP-specific phosphodiesterase HSPDE4D3 mutants which mimic activation and changes in rolipram inhibition triggered by protein kinase A phosphorylation of Ser-54: generation of a molecular model.
The Biochemical journal - 1 Jul 1998
Hoffmann R, Wilkinson I R, McCallum J F, Engels P, Houslay M D
Abstract excerpt
Ser-13 and Ser-54 were shown to provide the sole sites for the protein kinase A (PKA)-mediated phosphorylation of the human cAMP-specific phosphodiesterase isoform HSPDE4D3. The ability of PKA to phosphorylate and activate HSPDE4D3 was mimicked by replacing Ser-54 with either of the negatively ch...
Topics
- 3',5'-Cyclic-AMP Phosphodiesterases
- Animals
- Antigens, Viral
- COS Cells
- Cell Line, Transformed
- Cyclic AMP-Dependent Protein Kinases
- Enzyme Activation
- Epitopes
- Humans
- Models, Molecular
- Mutagenesis, Site-Directed