Article
Immobilized RNA switches for the analysis of complex chemical and biological mixtures.
Nature biotechnology - 1 Apr 2001
Seetharaman S, Zivarts M, Sudarsan N, Breaker R R
Abstract excerpt
A prototype biosensor array has been assembled from engineered RNA molecular switches that undergo ribozyme-mediated self-cleavage when triggered by specific effectors. Each type of switch is prepared with a 5'-thiotriphosphate moiety that permits immobilization on gold to form individually addressable pixels. The ribozymes comprising each pixel become active only when presented with their corresponding effector,...
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