Article
Tn10 insertional mutations of Bacillus subtilis that block the biosynthesis of bacilysin.
Biochimica et biophysica acta - 19 Mar 2001
Yazgan A, Ozcengiz G, Marahiel M A
Abstract excerpt
Transposon mutagenesis was employed to isolate the gene(s) related with the biosynthesis of dipeptide antibiotic in Bacillus subtilis PY79 (a prototrophic derivative of the standard 168 strain). The blocked mutants were phenotypically selected from the transposon library by bioassay and the complete loss of biosynthetic ability was verified through ESI-mass spectrometry analysis. Four different bacilysin...
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