Article
The basic loop of the RNase H domain of MLV RT is important both for RNase H and for polymerase activity.
Virology - 30 Mar 2001
Boyer P L, Gao H Q, Frank P, Clark P K, Hughes S H
Abstract excerpt
Escherichia coli RNase H has a basic extension that is involved in binding nucleic acid substrates. This basic extension is present in the RNase H of Moloney murine leukemia virus reverse transcriptase (MLV RT), but has been deleted from the RNase H of HIV-1 RT. Previous work showed that removing the basic loop from MLV RT (the mutant is called DeltaC) blocked viral replication; however, DeltaC MLV RT retained...
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