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An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics.

Environmental microbiology - 1 Feb 2000

Osborn A M, Moore E R, Timmis K N

Abstract excerpt

A systematic evaluation of the value and potential of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure has been undertaken. The reproducibility and robustness of the method has been assessed using environmental DNA samples isolated directly from PCB-polluted or pristine soil, and subsequent polymerase chain reaction (PCR) amplification of total...

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PMID: 11243261
DOI: 10.1046/j.1462-2920.2000.00081.x

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