Article
Generation and characterization of a PhoP homologue mutant of Neisseria meningitidis.
Molecular microbiology - 1 Mar 2001
Johnson C R, Newcombe J, Thorne S, Borde H A, Eales-Reynolds L J, Gorringe A R, Funnell S G, McFadden J J
Abstract excerpt
Two-component regulatory systems are important regulators of virulence genes in a number of bacteria. Genes encoding a two-component regulator system, with homology to the phoP/phoQ system in salmonella, were identified in the meningococcal genome. Allele replacement was used to generate a meningococcal knock-out mutant of the regulator component of this system, and its phenotype was examined. The mutant...
Topics
- Alleles
- Animals
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Blood Bactericidal Activity
- Blotting, Southern
- Epithelial Cells
- Gene Expression Regulation, Bacterial
- Humans
- Hydrogen-Ion Concentration
- Meningococcal Infections
- Mice
- Mutation