Article
Ambient pH signaling regulates nuclear localization of the Aspergillus nidulans PacC transcription factor.
Molecular and cellular biology - 1 Mar 2001
Mingot J M, Espeso E A, Díez E, Peñalva M A
Abstract excerpt
The Aspergillus nidulans zinc finger transcription factor PacC is activated by proteolytic processing in response to ambient alkaline pH. The pH-regulated step is the transition of full-length PacC from a closed to an open, protease-accessible conformation. Here we show that in the absence of ambient pH signaling, the C-terminal negative-acting domain prevents the nuclear localization of full-length closed PacC....
Topics
- Amino Acids
- Aspergillus nidulans
- Blotting, Western
- Cell Nucleus
- Cytoplasm
- Cytosol
- DNA
- DNA-Binding Proteins
- Fungal Proteins
- Green Fluorescent Proteins
- Hydrogen-Ion Concentration