Article
Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA.
The Journal of molecular diagnostics : JMD - 1 Aug 2000
Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M
Abstract excerpt
We developed a homogeneous format reverse transcription-polymerase chain reaction assay for quantitating hepatitis C virus (HCV) RNA based on the TaqMan principle, in which signal is generated by cleaving a target-specific probe during amplification. The test uses two probes, one specific for HCV and one specific for an internal control, containing fluorophores with different emission spectra. Titers are...
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