Article
TaqMan amplification system with an internal positive control for HCV RNA quantitation.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Nov 2004
Castelain Sandrine, Descamps Véronique, Thibault Vincent, François Catherine, Bonte Dorine, Morel Virginie, Izopet Jacques, Capron Dominique, Zawadzki Patricia, Duverlie Gilles
Abstract excerpt
BACKGROUND: Quantitation of hepatitis C virus (HCV) RNA has become an essential tool for monitoring antiviral therapies in chronically infected patients. Different quantitative HCV RNA assays have been reported, mainly using techniques based on signal amplification with branched DNA (bDNA) technology or target sequence amplification by reverse-transcription PCR method (RT-PCR). OBJECTIVES AND STUDY DESIGN: An...
Topics
- Genotype
- Hepacivirus
- Hepatitis C
- Humans
- RNA, Viral
- Reference Standards
- Reproducibility of Results
- Reverse Transcriptase Polymerase Chain Reaction
- Sensitivity and Specificity