Article
A novel cell culture model of chondrocyte differentiation during mammalian endochondral ossification.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Feb 2001
Cheung J O, Hillarby M C, Ayad S, Hoyland J A, Jones C J, Denton J, Thomas J T, Wallis G A, Grant M E
Abstract excerpt
Endochondral ossification (EO) occurs in the growth plate where chondrocytes pass through discrete stages of proliferation, maturation, hypertrophy, and calcification. We have developed and characterized a novel bovine cell culture model of EO that mirrors these events and will facilitate in vitro studies on factors controlling chondrocyte differentiation. Chondrocytes derived from the epiphyses of long bones of...
Topics
- Animals
- Azacitidine
- Base Sequence
- Bone and Bones
- Calcification, Physiologic
- Cattle
- Cells, Cultured
- Chondrocytes
- DNA Primers
- Immunohistochemistry
- Models, Biological
- Parathyroid Hormone-Related Protein
- Phenotype