Article
Cloning of the Aspergillus oryzae 5-aminolevulinate synthase gene and its use as a selectable marker.
Current genetics - 1 Dec 2000
Elrod S L, Jones A, Berka R M, Cherry J R
Abstract excerpt
The hemA gene encoding 5-aminolevulinate synthase, the first enzyme in heme biosynthesis, was cloned from Aspergillus oryzae and evaluated as a selectable marker for the transformation of filamentous fungi. Deletion of the hemA gene resulted in a lethal phenotype that could be rescued either by the supplementation of culture media with 5-aminolevulinic acid (ALA) or by transformation with the wild-type hemA gene,...
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