Article
HIV type 2 Vpx interaction with Gag and incorporation into virus-like particles.
AIDS research and human retroviruses - 20 Jan 2001
Jin L, Zhou Y, Ratner L
Abstract excerpt
The domain of HIV-2 Vpx previously shown to be important for virion incorporation has been mapped to residues 73--89. Mutational analysis of this domain was employed to further define the sequences important for incorporation into virus-like particles, using a vaccinia virus expression system. Deletion of residues 73--89 did not abrogate Vpx packaging, but substitution with alanines markedly reduced incorporation...
Topics
- Alanine
- Amino Acid Sequence
- Amino Acid Substitution
- Autoradiography
- Cell Line
- Electrophoresis, Polyacrylamide Gel
- Fusion Proteins, gag-pol
- Gene Deletion
- Gene Products, gag
- Glutathione Transferase
- HIV-2
- Humans
- Immunoblotting
- Molecular Sequence Data
- Mutation
- Viral Regulatory and Accessory Proteins