Article
Multiplex allele-specific target amplification based on PCR suppression.
Proceedings of the National Academy of Sciences of the United States of America - 2 Jan 2001
Broude N E, Zhang L, Woodward K, Englert D, Cantor C R
Abstract excerpt
We have developed a strategy for multiplex PCR based on PCR suppression. PCR suppression allows DNA target amplification with only one sequence-specific primer per target and a second primer that is common for all targets. Therefore, an n-plex PCR would require only n + 1 primers. We have demonstrated uniform, efficient amplification of targeted sequences in 14-plex PCR. The high specificity of suppression PCR...
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