Article
Vibrio vulnificus RTX toxin kills host cells only after contact of the bacteria with host cells.
Cellular microbiology - 1 Apr 2008
Kim Young Ran, Lee Shee Eun, Kook Hyun, Yeom Jung A, Na Hee Sam, Kim Soo Young, Chung Sun Sik, Choy Hyon E, Rhee Joon Haeng
Abstract excerpt
Vibrio vulnificus causes acute cell death and a fatal septicaemia. In this study, we show that contact with host cells is a prerequisite to the acute cytotoxicity. We screened transposon mutants defective in the contact-dependent cytotoxicity. Two mutants had insertions within two open reading frames in a putative RTX toxin operon, the rtxA1 or rtxD encoding an RTX toxin (4701 amino acids) or an ABC type...
Topics
- Animals
- Bacterial Toxins
- Cell Death
- Chloramphenicol
- DNA Transposable Elements
- Female
- Genetic Complementation Test
- HeLa Cells
- Hemolysis
- Host-Pathogen Interactions
- Humans
- Mice
- Microscopy, Confocal
- Mutagenesis, Insertional