Article
Preincubation with endothelial cell monolayers increases gene transfer efficiency into human bone marrow CD34(+)CD38(-) progenitor cells.
Human gene therapy - 10 Dec 2000
Chute J P, Saini A, Wells M, Clark W, Wu A, St Louis D, Blair P, Harlan D, Kaushal S
Abstract excerpt
Retroviral gene transfer studies targeting bone marrow CD34(+)CD38(-) stem cells have been disappointing because of the rarity of these cells, their G(0) cell cycle status, and their low or absent expression of surface retroviral receptors. In this study, we examined whether preincubation of bone marrow CD34(+)CD38(-) stem cells with a hematopoietically supportive porcine microvascular endothelial cell line...
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