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Article

Sequential action of two GTPases to promote vacuole docking and fusion.

The EMBO journal - 15 Dec 2000

Eitzen G, Will E, Gallwitz D, Haas A, Wickner W

Abstract excerpt

Homotypic vacuole fusion occurs by sequential priming, docking and fusion reactions. Priming frees the HOPS complex (Vps 11, 16, 18, 33, 39 and 41) to activate Ypt7p for docking. Here we explore the roles of the GDP and GTP states of Ypt7p using Gdi1p (which extracts Ypt7:GDP), Gyp7p (a GTPase-activating protein for Ypt7p:GTP), GTPgammaS or GppNHp (non-hydrolyzable nucleotides), and mutant forms of Ypt7p that...

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Identifiers

PMID: 11118206
DOI: 10.1093/emboj/19.24.6713
PMCID: PMC305897

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Sequential action of two GTPases to promote vacuole docking and fusion.PMID 11118206Read ↗

Rho1p and Cdc42p act after Ypt7p to regulate vacuole docking.Semantic proximity Read ↗

The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance.Semantic proximity Read ↗

Docking of yeast vacuoles is catalyzed by the Ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF).Semantic proximity Read ↗

Vps41 phosphorylation and the Rab Ypt7 control the targeting of the HOPS complex to endosome-vacuole fusion sites.Semantic proximity Read ↗

Guanine nucleotide exchange factors (GEFs) have a critical but not exclusive role in organelle localization of Rab GTPases.Semantic proximity Read ↗

New component of the vacuolar class C-Vps complex couples nucleotide exchange on the Ypt7 GTPase to SNARE-dependent docking and fusion.Semantic proximity Read ↗

The docking of primed vacuoles can be reversibly arrested by excess Sec17p (alpha-SNAP).Semantic proximity Read ↗

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