Article
Telomere-binding and Stn1p-interacting activities are required for the essential function of Saccharomyces cerevisiae Cdc13p.
Nucleic acids research - 1 Dec 2000
Wang M J, Lin Y C, Pang T L, Lee J M, Chou C C, Lin J J
Abstract excerpt
Yeast Saccharomyces cerevisiae Cdc13p is the telomere-binding protein that protects telomeres and regulates telomere length. It is documented that Cdc13p binds specifically to single-stranded TG(1-3) telomeric DNA sequences and interacts with Stn1p. To localize the region for single-stranded TG(1-3) DNA binding, Cdc13p mutants were constructed by deletion mutagenesis and assayed for their binding activity. Based...
Topics
- Cyclin B
- DNA, Single-Stranded
- DNA-Binding Proteins
- Fungal Proteins
- Genetic Complementation Test
- Mutation
- Peptide Fragments
- Protein Binding
- Saccharomyces cerevisiae
- Telomere
- Two-Hybrid System Techniques