Article
De novo synthesis of minus strand RNA by the rotavirus RNA polymerase in a cell-free system involves a novel mechanism of initiation.
RNA (New York, N.Y.) - 1 Oct 2000
Chen D, Patton J T
Abstract excerpt
The replicase activity of rotavirus open cores has been used to study the synthesis of (-) strand RNA from viral (+) strand RNA in a cell-free replication system. The last 7 nt of the (+) strand RNA, 5'-UGUGACC-3', are highly conserved and are necessary for efficient (-) strand synthesis in vitro. Characterization of the cell-free replication system revealed that the addition of NaCl inhibited (-) strand...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.