Article
Analysis of upstream elements in the HuC promoter leads to the establishment of transgenic zebrafish with fluorescent neurons.
Developmental biology - 15 Nov 2000
Park H C, Kim C H, Bae Y K, Yeo S Y, Kim S H, Hong S K, Shin J, Yoo K W, Hibi M, Hirano T, Miki N, Chitnis A B, Huh T L
Abstract excerpt
HuC encodes an RNA binding protein homologous to Drosophila elav that serves as an excellent early marker for differentiating neurons. We have characterized the promoter of the zebrafish HuC gene by examining the ability of 5'-upstream fragments to drive expression of green fluorescent protein (GFP) in live embryos. We determined that 2.8 kb of the 5'-flanking sequence is sufficient to restrict GFP gene...
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