Article
Context-dependent EKLF responsiveness defines the developmental specificity of the human epsilon-globin gene in erythroid cells of YAC transgenic mice.
Genes & development - 1 Nov 2000
Tanimoto K, Liu Q, Grosveld F, Bungert J, Engel J D
Abstract excerpt
We explored the mechanism of definitive-stage epsilon-globin transcriptional inactivity within a human beta-globin YAC expressed in transgenic mice. We focused on the globin CAC and CAAT promoter motifs, as previous laboratory and clinical studies indicated a pivotal role for these elements in globin gene activation. A high-affinity CAC-binding site for the erythroid krüppel-like factor (EKLF) was placed in the...
Topics
- Age Factors
- Animals
- Binding Sites
- COUP Transcription Factors
- Chromosome Inversion
- DNA-Binding Proteins
- Erythroid Precursor Cells
- Gene Expression Regulation, Developmental
- Globins
- Humans
- K562 Cells
- Kruppel-Like Transcription Factors
- Leukemia, Erythroblastic, Acute