Article
Conditions for conjugative transposon transfer in Lactococcus lactis.
Letters in applied microbiology - 1 Nov 2000
Blaiotta G, Ercolini D, Simeoli E, Moschetti G, Villani F
Abstract excerpt
Three different techniques for bacterial mating were applied to wild type and culture collection strains of Lactococcus lactis harbouring transposons: direct plate conjugation, filter mating and mating on milk agar. Efficiencies and frequencies of transfer were compared. Transconjugants were characterized by marker properties and molecular assays. Transposon-coded Suc+ Nis+ phenotype as well as Suc+ Bac+ Nis-...
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