Conditions for conjugative transposon transfer in Lactococcus lactis.

doi:10.1046/j.1472-765x.2000.00801.x

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Article

Conditions for conjugative transposon transfer in Lactococcus lactis.

Letters in applied microbiology - 1 Nov 2000

Blaiotta G, Ercolini D, Simeoli E, Moschetti G, Villani F

Abstract excerpt

Three different techniques for bacterial mating were applied to wild type and culture collection strains of Lactococcus lactis harbouring transposons: direct plate conjugation, filter mating and mating on milk agar. Efficiencies and frequencies of transfer were compared. Transconjugants were characterized by marker properties and molecular assays. Transposon-coded Suc+ Nis+ phenotype as well as Suc+ Bac+ Nis-...

Topics

Conjugation, Genetic
Crosses, Genetic
DNA Transposable Elements
Dairy Products
Electrophoresis, Gel, Pulsed-Field
Food Microbiology
Lactococcus lactis
Phenotype

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Open in PubMed

Identifiers

PMID: 11069634
DOI: 10.1046/j.1472-765x.2000.00801.x

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