Article
Insertion of Tn916 into Bacillus pumilus plasmid pMGD302 and evidence for plasmid transfer by conjugation.
Plasmid - 1 Jul 1991
Hendrick C A, Johnson L K, Tomes N J, Smiley B K, Price J P
Abstract excerpt
As part of an effort to develop systems for genetic analysis of strains of Bacillus pumilus which are being used as a microbial hay preservative, we introduced the conjugative Enterococcus faecalis transposon Tn916 into B. pumilus ATCC 1 and two naturally occurring hay isolates of B. pumilus. B. pumilus transconjugants resistant to tetracycline were detected at a frequency of approximately 6.5 x 10(-7) per...
Topics
- Bacillus
- Blotting, Southern
- Conjugation, Genetic
- Crosses, Genetic
- DNA Transposable Elements
- DNA, Bacterial
- Enterococcus faecalis
- Phenotype
- Plasmids