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RaSH, a rapid subtraction hybridization approach for identifying and cloning differentially expressed genes

31 Oct 2000

Abstract excerpt

Human melanoma cells growth-arrest irreversibly and terminally differentiate on treatment with a combination of fibroblast interferon and the protein kinase C activator mezerein. This experimental protocol also results in a loss of tumorigenic potential and profound changes in gene expression. Various cloning and cDNA microarray strategies are being used to determine the complete spectrum of gene expression...

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PMID: 11058161
DOI: 10.1073/pnas.220431297

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