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Rapid interactome profiling by massive sequencing

9 Feb 2010

Abstract excerpt

We have developed a high-throughput protein expression and interaction analysis platform that combines cDNA phage display library selection and massive gene sequencing using the 454 platform. A phage display library of open reading frame (ORF) fragments was created from mRNA derived from different tissues. This was used to study the interaction network of the enzyme transglutaminase 2 (TG2), a multifunctional...

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PMID: 20144949
DOI: 10.1093/nar/gkq052

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