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Article

Identification of sample-specific sequences in mammalian cDNA and genomic DNA by the novel ligation-mediated subtraction (Limes).

Nucleic acids research - 15 Feb 2001

Hansen-Hagge T E, Trefzer U, zu Reventlow A S, Kaltoft K, Sterry W

Abstract excerpt

The representational difference analysis (RDA) and other subtraction techniques are used to enrich sample-specific sequences by elimination of ubiquitous sequences existing in both the sample of interest (tester) and the subtraction partner (driver). While applying the RDA to genomic DNA of cutaneous lymphoma cells in order to identify tumor relevant alterations, we predominantly isolated repetitive sequences and...

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Identifiers

PMID: 11160940
DOI: 10.1093/nar/29.4.e20
PMCID: PMC29627

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