Article
Structural and functional similarities between HIV-1 reverse transcriptase and the Escherichia coli RNA polymerase beta' subunit.
FEBS letters - 27 Oct 2000
Szilvay A M, Stern B, Blichenberg A, Helland D E
Abstract excerpt
Four monoclonal antibodies (MAbs) recognizing HIV-1 reverse transcriptase (RT) were shown here to cross-react with the beta' subunit of Escherichia coli RNA polymerase (RNAP). The anti-RT MAbs bind to a peptide comprising residues 294-305 of the RT amino acid sequence. Computer analyses revealed sequence similarity between this peptide and two regions of the RNAP beta' subunit. MAb-binding studies using RT...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Binding Sites
- Blotting, Western
- Catalysis
- Cross Reactions
- DNA-Directed RNA Polymerases
- Dose-Response Relationship, Drug
- Epitopes
- Escherichia coli
- HIV Reverse Transcriptase
- HIV-1
- Kinetics
- Molecular Sequence Data