Article
Structure and function of microplasminogen: reconstitution of microplasminogen and microplasmin from isolated fragments.
Ciba Foundation symposium - 1 Jan 1997
de los Santos T, Wang J, Reich E
Abstract excerpt
We describe limited chemical proteolysis of microplasminogen/microplasmin (mPlg/mPlm) and their reconstitution from isolated fragments. A V141-->M141 substitution in methionineless human mPlg/mPlm allowed the protein(s) to be cleaved in CNBr/formic acid. The resulting two fragments (141 and 118 residues, respectively), each internally disulfide bonded, were separated by preparative non-reducing gradient SDS-PAGE,...
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