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Article

The role of the glycine triad in human glutathione synthetase.

Biochemical and biophysical research communications - 1 Oct 2010

Dinescu Adriana, Brown Teresa R, Barelier Sarah, Cundari Thomas R, Anderson Mary E

Abstract excerpt

Experimental kinetics and computational modeling of human glutathione synthetase (hGS) support the significant role of the G-loop glycine triad (G369, G370, G371) for activity of this ATP-grasp enzyme. Enzyme kinetic experiments indicate that G369V and G370V mutant hGS have little activity (<0.7 and 0.3%, respectively, versus wild-type hGS). However, G371V retains ∼13% of the activity of wild-type hGS. With...

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Identifiers

PMID: 20800579
DOI: 10.1016/j.bbrc.2010.08.081
PMCID: PMC3065366

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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