Article
Cloning and functional characterization of a 30 kb gene locus required for lipopolysaccharide biosynthesis in Legionella pneumophila.
International journal of medical microbiology : IJMM - 1 Mar 2000
Lüneberg E, Zetzmann N, Alber D, Knirel Y A, Kooistra O, Zähringer U, Frosch M
Abstract excerpt
The spontaneous Legionella pneumophila lipopolysaccharide (LPS) mutant 137, which did not bind the LPS-specific mAb 2625, was complemented with a genomic library from the parental wild-type strain. Transformants were screened for reconstitution of the wild-type LPS phenotype, able to bind mAb 2625. By this strategy, a 32,661 bp region comprising 30 open reading frames (Orfs) was identified. Orfs with significant...
Topics
- Blotting, Western
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Genes, Bacterial
- Genetic Complementation Test
- Humans
- Legionella pneumophila
- Lipopolysaccharides
- Mutation
- Open Reading Frames
- Sequence Analysis, DNA
- Structure-Activity Relationship
- Virulence