Article
Genetic analysis of the O7-polysaccharide biosynthesis region from the Escherichia coli O7:K1 strain VW187.
Journal of bacteriology - 1 Jul 1990
Marolda C L, Welsh J, Dafoe L, Valvano M A
Abstract excerpt
We recently cloned biosynthesis genes for the O7-lipopolysaccharide (O7-LPS) side chain from the Escherichia coli K-1 strain VW187 (M. A. Valvano, and J. H. Crosa, Infect. Immun. 57:937-943, 1989). To characterize the O7-LPS region, the recombinant cosmids pJHCV31 and pJHCV32 were mutagenized by transposon mutagenesis with Tn3HoHo1, which carries a promoterless lac operon and can therefore generate lacZ...
Topics
- Chromosome Mapping
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Recombinant
- Escherichia coli
- Genes, Bacterial
- Lipopolysaccharides
- Mutation
- Plasmids
- beta-Galactosidase
