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Article

Genetic analysis of the O7-polysaccharide biosynthesis region from the Escherichia coli O7:K1 strain VW187.

Journal of bacteriology - 1 Jul 1990

Marolda C L, Welsh J, Dafoe L, Valvano M A

Abstract excerpt

We recently cloned biosynthesis genes for the O7-lipopolysaccharide (O7-LPS) side chain from the Escherichia coli K-1 strain VW187 (M. A. Valvano, and J. H. Crosa, Infect. Immun. 57:937-943, 1989). To characterize the O7-LPS region, the recombinant cosmids pJHCV31 and pJHCV32 were mutagenized by transposon mutagenesis with Tn3HoHo1, which carries a promoterless lac operon and can therefore generate lacZ...

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Identifiers

PMID: 2163382
DOI: 10.1128/jb.172.7.3590-3599.1990
PMCID: PMC213332

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