Pseudomonas aeruginosa galU is required for a complete lipopolysaccharide core and repairs a secondary mutation in a PA103 (serogroup O11) wbpM mutant.

Abstract excerpt

Insertional inactivation of wbpM in Pseudomonas aeruginosa serogroup O11 strain PA103 resulted in mutants exhibiting three distinct lipopolysaccharide (LPS) phenotypes. One mutant, PA103 wbpM-C, had a truncated LPS core and lacked O antigen. These defects were not complemented by the cloned wbpM gene, suggesting a secondary mutation was present. When the wild-type galU gene was introduced into strain PA103 wbpM-C...

Topics

• Bacterial Proteins
• Blotting, Western
• Electrophoresis, Polyacrylamide Gel
• Escherichia coli Proteins
• Genetic Complementation Test
• Hydro-Lyases
• Lipopolysaccharides
• Mutagenesis, Insertional
• Mutation
• O Antigens