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Article

Resistance to methotrexate due to AcrAB-dependent export from Escherichia coli.

Antimicrobial agents and chemotherapy - 1 Nov 2000

Kopytek S J, Dyer J C, Knapp G S, Hu J C

Abstract excerpt

Many laboratory strains of Escherichia coli are resistant to methotrexate (MTX), a folate analogue that binds dihydrofolate reductase (DHFR). Mutations that inactivate either tolC or acrA confer MTX sensitivity. Further, overexpression of a fusion protein with DHFR activity reverses this sensitivity by titrating out intracellular MTX. These results suggest that MTX accumulates in cells where mutations in acrA or...

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Share to ATP Binding Cassette Transporter, Subfamily B, Member 1
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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 11036056
DOI: 10.1128/AAC.44.11.3210-3212.2000
PMCID: PMC101636

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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