Article
Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrity.
Molecular and cellular biology - 1 Nov 2000
Snaith H A, Brown G W, Forsburg S L
Abstract excerpt
The fission yeast Hsk1p kinase is an essential activator of DNA replication. Here we report the isolation and characterization of a novel mutant allele of the gene. Consistent with its role in the initiation of DNA synthesis, hsk1(ts) genetically interacts with several S-phase mutants. At the restrictive temperature, hsk1(ts) cells suffer abnormal S phase and loss of nuclear integrity and are sensitive to both...
Topics
- Alleles
- Amino Acid Sequence
- Bleomycin
- Cell Cycle Proteins
- Cell Nucleus
- Checkpoint Kinase 2
- DNA Replication
- Dose-Response Relationship, Radiation
- Fluorescent Antibody Technique
- Fungal Proteins
- G1 Phase