Article
Rapid genotyping of mouse tissue using Sigma's Extract-N-Amp Tissue PCR Kit.
Journal of visualized experiments : JoVE - 22 Jan 2008
Doan Linda, Monuki Edwin S
Abstract excerpt
Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is determined. Conventional preparation of tissues for PCR-ready DNA often take several hours to days, depending on the tissue sample. The genotype of the sample may thus be delayed for several days, which is not an option for many different types of experiments. Here we...
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