Article
Characterization of a monoclonal antibody directed to the surface of MA104 cells that blocks the infectivity of rotaviruses.
Virology - 20 Jul 2000
López S, Espinosa R, Isa P, Merchant M T, Zárate S, Méndez E, Arias C F
Abstract excerpt
Rhesus rotavirus (RRV) binds to sialic acid residues on the surface of target cells, and treatment of these cells with neuraminidase greatly reduces virus binding with the consequent reduction of infectivity. Variants that can efficiently infect neuraminidase-treated cells have been isolated, indicating that attachment to sialic acid is not an essential step for animal rotaviruses to infect cells. To identify and...
Topics
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Biotinylation
- Cell Line
- Epitopes
- Flow Cytometry
- Fluorescent Antibody Technique, Indirect
- Genetic Variation
- Humans
- Hybridomas
- Macaca mulatta
- Mice
- Microscopy, Immunoelectron