Article
A model for the PI-SceIxDNA complex based on multiple base and phosphate backbone-specific photocross-links.
Journal of molecular biology - 21 Jul 2000
Christ F, Steuer S, Thole H, Wende W, Pingoud A, Pingoud V
Abstract excerpt
We have synthesized different oligodeoxynucleotides carrying, in single positions of the >36 bp recognition site of PI-SceI, photoreactive base analogues (5-iododeoxypyrimidines) or phosphate modifications (p-azidophenacylphosphorothioates) and used them in photocross-linking experiments with PI-SceI to probe the protein-DNA interface of the specific complex between the homing endonuclease PI-SceI and its DNA...
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