Article
Ordered membrane insertion of an archaeal opsin in vivo.
Proceedings of the National Academy of Sciences of the United States of America - 5 Jul 2000
Dale H, Angevine C M, Krebs M P
Abstract excerpt
The prevailing model of polytopic membrane protein insertion is based largely on the in vitro analysis of polypeptide chains trapped during insertion by arresting translation. To test this model under conditions of active translation in vivo, we have used a kinetic assay to determine the order and timing with which transmembrane segments of bacterioopsin (BO) are inserted into the membrane of the archaeon...
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