Article
Wild-type and mutant bacterioopsins D85N, D96N, and R82Q: high-level expression in Escherichia coli.
Biochemistry - 26 Mar 1991
Shand R F, Miercke L J, Mitra A K, Fong S K, Stroud R M, Betlach M C
Abstract excerpt
The integral membrane protein bacterioopsin, found in the extremely halophilic archaebacterium Halobacterium halobium, was expressed in Escherichia coli as a fusion protein containing 13 heterologous amino acids at the amino terminus. The expressed protein was localized primarily to the E. coli cytoplasmic membrane (greater than 80%) and had an in vivo half-life of 26 min. The amount of bacterioopsin in E. coli...
Topics
- Amino Acid Sequence
- Autoradiography
- Bacteriorhodopsins
- Base Sequence
- Blotting, Western
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genetic Vectors
- Half-Life
- Halobacterium