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Article

Membrane insertion kinetics of a protein domain in vivo. The bacterioopsin n terminus inserts co-translationally.

The Journal of biological chemistry - 6 Aug 1999

Dale H, Krebs M P

Abstract excerpt

The pathway by which segments of a polytopic membrane protein are inserted into the membrane has not been resolved in vivo. We have developed an in vivo kinetic assay to examine the insertion pathway of the polytopic protein bacterioopsin, the apoprotein of Halobacterium salinarum bacteriorhodopsin. Strains were constructed that express the bacteriorhodopsin mutants I4C:H(6) and T5C:H(6), which carry a unique Cys...

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Identifiers

PMID: 10428851
DOI: 10.1074/jbc.274.32.22693

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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