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Article

Isolation and characterization of polyadenylation complexes assembled in vitro.

RNA (New York, N.Y.) - 1 May 2000

Veraldi K L, Edwalds-Gilbert G, MacDonald C C, Wallace A M, Milcarek C

Abstract excerpt

We developed a two-step purification of mammalian polyadenylation complexes assembled in vitro. Biotinylated pre-mRNAs containing viral or immunoglobulin poly(A) sites were incubated with nuclear extracts prepared from mouse myeloma cells under conditions permissive for in vitro cleavage and polyadenylation and the mixture was fractionated by gel filtration; complexes containing biotinylated pre-mRNA and bound...

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Identifiers

PMID: 10836797
DOI: 10.1017/s135583820099246x
PMCID: PMC1369956

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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