Article
Role of coatomer and phospholipids in GTPase-activating protein-dependent hydrolysis of GTP by ADP-ribosylation factor-1.
The Journal of biological chemistry - 4 Aug 2000
Szafer E, Pick E, Rotman M, Zuck S, Huber I, Cassel D
Abstract excerpt
The binding of the coat protein complex, coatomer, to the Golgi is mediated by the small GTPase ADP-ribosylation factor-1 (ARF1), whereas the dissociation of coatomer, requires GTP hydrolysis on ARF1, which depends on a GTPase-activating protein (GAP). Recent studies demonstrate that when GAP activity is assayed in a membrane-free environment by employing an amino-terminal truncation mutant of ARF1 (Delta17-ARF1)...
Topics
- ADP-Ribosylation Factor 1
- Arginine
- Carrier Proteins
- Coatomer Protein
- GTPase-Activating Proteins
- Guanosine Triphosphate
- Hydrolysis
- Mutation
- Myristic Acid
- Phospholipids
- Protein Processing, Post-Translational
- Sequence Deletion